Scientist Flow Cytometry (mfd)

I Indivumed is a biotech company focusing on precision oncology. For almost 25 years we have been driven by a single overarching vision : a world in which precision therapy offers a cure for every cancer patient.

Specialized in early drug discovery our R&D approach is based on the belief that we need to fundamentally understand the disease to gain novel insights and develop effective therapeutics. To do so we start with carefully collected and curated patient samples resulting in a comprehensive and proprietary database. By employing advanced biomathematical and bioinformatic methods on this excellent data we discover therapeutically novel targets. From our high-quality tissue we generate patient-derived 2D and 3D cellular models allowing us to perform target validation and therapeutic development in an environment that is as close to the patient as possible.

Drive dedication and our shared purpose make us who we are.

Driven by our pioneering spirit and our shared purpose we explore the complexity of cancer with curiosity and determination. Thriving on innovation we value unique perspectives and visionary scientific approaches to grow together. Whether as a scientist as a data analyst or in a corporate function everyone contributes to our common goal.

If you thrive on discovery come join our team and make your impact!

Tasks

We are looking for an exceptional Scientist to lead our efforts in the identification and characterization of novel therapeutic antibodies. You will be the subject matter expert for flow cytometry responsible for standardizing executing and analyzing high-throughput screening of hybridomas targeting critical cancer surface proteins.

• Serve as the teams go-to expert for complex multi-color flow cytometry (BD Fortessa X20)
• Perform daily instrument quality control calibration compensation and troubleshooting to ensure consistent high-quality data output.
• Develop and implement SOPs. Train other users.
• Establish SOPs for panel design titration of antibodies sample preparation staining acquisition and data analysis using FlowJo or similar software.
• Design optimize and implement robust high-throughput flow cytometry-based screening protocols to identify and rank monoclonal antibodies from hybridomas.
• Flow cytometry-based analysis of receptor internalization / shedding
• Independently analyze datasets using software such as FlowJo. Interpret results critically and present findings.
• Develop and execute high throughput flow-based assays including immunophenotyping cell viability cell cycle and cytokine profiling.
• Develop validate and perform a range of cancer-specific immunological and cell-based functional assays to characterize antibody candidates such as CDC and ADCC
• Work collaboratively with colleagues in all departments to develop flow cytometry-based assays
• Maintain accurate documentation of experiments and service logs for compliance and reproducibility.

Requirements

• PhD in Immunology Cancer Biology Cell Biology or a related discipline.
• Solid understanding of flow cytometry principles sample preparation and multi-color panel optimization. Extensive hands-on experience with flow cytometry including panel design compensation multicolor analysis and complex gating strategies troubleshooting maintenance of the flow cytometer.
• Experience with high throughput FACS acquisition and analysis at 96 / 384 well scale is required
• Proven expertise in working with monoclonal antibody characterization against cell surface targets and analysis of receptor internalization / shedding.
• Solid understanding of cancer immunology and experience developing functional assays such as ADCC CDC or immune checkpoint assays.
• Experience with immuno-histochemistry is preferred.
• Excellent organizational skills and attention to detail with a proven ability to manage multiple tasks and deliver results to deadlines.
• Clear and effective communication skills (both written and verbal) and the ability to work effectively in a team-oriented environment.

Benefits

• Demanding area of responsibility in a fast-growing company
• Grant for the HVV ProfiTicket / Deutschlandticket
• Subsidy for sports membership and events
• Company pension scheme
• Company doctor
• Fresh fruits and drinks
• Christmas and summer party
• Flexible working hours
• German and English courses
• 30 days holiday
• The 24th and 31st of December are holidays and wont be deducted from your annual holidays.

Key Skills

• Laboratory Experience
• Immunoassays
• Machine Learning
• Biochemistry
• Assays
• Research Experience
• Spectroscopy
• Research & Development
• cGMP
• Cell Culture
• Molecular Biology
• Data Analysis Skills

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