Technical Assistant (d/f/m)

The candidate will join the ARPA-H project LIVE which aims to bioprint an entire human liver for transplantation in the context of acute liver disease. The role of the candidate will be (i) to identify and to characterise GMP grade human induced Pluripotent Stem Cell line suitable for the project, (ii) to produce, to charactersie and to quality control liver cells at large quantity for the project. For that, they will develop new methods for to differentiation hiPSC lines using bioreactor. The resulting cell will be then used to bioprint artificial liver in vitro in collaboration without the international network of collaborators of the ARPA-H LIVE program.

Responsibilities

• Cell culture production (grow, characterise, differentiate human pluripotent stem cells)
• Application of previously determined analytical procedures
• Filling, operation and calibration/programming of the instruments to be used
• Evaluation and interpretation of the analysis results with regard to plausibility and quality
• Receipt, documentation and tracking of valuable samples
• Review and qualitative evaluation of the sample
• Technical elaboration of the experimental setup
• Maintenance of laboratory equipment, if necessary organization of external service and repair measures.
• Supervision of general laboratory operations and organization
• Maintenance of the laboratory workplace, taking into account occupational health and safety and disposal guidelines
• Ordering and stocking, e.g. of consumables, media and solutions
• Performance of analytical procedures (application of previously determined analytical procedures,filling, operation and calibration/programming of the instruments to be used, evaluation and interpretation of the analysis results with regard to plausibility and quality)

Qualifications

• Bachelor or equivalent in cell biology, bioengineering or regenerative medicine.
• Experience in tissue culture especially with human Pluripotent Stem Cells.
• Basic experience in flow cytometry, QPCR, and confocal imaging.
• Experience in working independently while keeping a strong team spirit.
• Strong self-motivation, organizational skills, and ability to lead and develop scientific projects.
• Scientific curiosity, analytical thinking, and interest in contributing to projects.
• Ability to work in a team of young scientists, to be flexible, and flourish in a fast-paced environment.
• Strong communication and interpersonal skills, ability to work collaborate effectively with individuals of different backgrounds in a multidisciplinary team, team orientated with excellent organizational skills including project management and good record keeping important.
• Fluent English

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